Hedrick Lab Protocols

Here are a few of the protocols used in the Hedrick Lab:

  • Bone Marrow Chimeras
  • CFSE Staining
  • Cytotoxicity Assays
  • DNA Fragmentation Assays for Apoptosis
  • ES Cell DNA Extraction: Tube Method
  • Fill-In Reactions
  • Low Density Spleen Prep
  • Mouse Monocycte Cell Lines
  • One-Step Miniprep for Screening by Size
  • PCR Method for Genotyping Perforin-Deficient Mice
  • Phenol/Chloroform Precipitation of DNA
  • Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.)
  • Tissue Culture: Splitting Cells
  • Transformation Protocol For DH5 Alpha
  • Transformation Protocol For MC1061/P3
  • Tail Chop Southern Protocol
  • Tail Preps: DNA Isolation From Mouse Tails Without Phenol
  • Erk1-/- mouse typing Protocol
  • Erk2fn/fn mouse typing Protocol

    Hedrick Lab Disclaimer:     These protocols may not be completely accurate.
    Questions or comments please e-mail Dr. Hedrick.